Allegedly peptides are so finicky that you may only store them right at the back of the fridge.
Therefore here is the research on SEMAX Stability – a great substance that releases BDNF and regenerates the brain.
Dr. Dean Berman
this peptide expert, who also heads the university peptide training program in Geneva, told us how he came to peptides:
amazing improvement of very old lady
As a young doctor he worked in a nursing home / care home and there met an old Russian woman. She was relatively pre-demented and 80 years old and in a rather poor condition.
When he visited this home again 10 years later, he found this patient significantly improved, rejuvenated and above all agile and full of life. She was by then in such good condition and at the same time so bored that, at the age of 90, she had begun working in the nursing home herself.
When he asked in total astonishment what had happened that she had regenerated so fantastically, she answered that she had obtained peptides from Russia, which have been in use there for a long time and are available in every pharmacy and are part of regular russian allopathic medicine.
In fact the peptides come from Russian research, and especially SEMAX is routinely used there with success in strokes.
BPC-157 repairs my hip joint
Unfortunately, as a physician one is not allowed to use “peptides” on patients because they have no approval,
but in a heroic self-experiment I can report that my hip, which was severely impaired by a labral tear as well as subchondral cysts of the joint cartilage and for 3 years allowed me no more than a limping walking distance of 200 m, was – honestly speaking – completely healed with BPC-157 within a few weeks.
No wonder that BPC-157 is banned as a DOPING AGENT!
where for a long time I could barely walk down from the 1st living-floor to the practice
for almost 3 years I was already limping so badly. I remember when I met Petros the first time i was severely affected by the pain, that lasted until last fall.
PRP injected into the hip joint improved the pain by about 50 % for only 3 months, then it was back to how it was before.
Severe improvement I got by using TPS 2 x – but after dancing at a party I got again pain.
Until I put only ONCE BPC-157 to my hip!
the proof is the performance capacity …
Last weekend I walked down a mountain about 400 meters of altitude, with my 110 kg live weight, 1 hour of steep downhill.
Despite severe muscle ache, the next day I rode the e-bike 10 km up the mountain road with 8 % incline and 800 meters of altitude and had no complaints at all, not even a sensation in the organ. Presumably boron also contributed.
everything heals “magically”
When an implant started to fester out 3 weeks ago, I immediately applied BPC-157 there and was able to eat normally one hour later, and the next day I noticed a considerable improvement in concentration ability, perhaps also because the “dental focus” was finally out.
Neuropeptide SEMAX considerably improves my performance capacity
I am just as enthusiastic about the neuropeptide SEMAX, I can now type 20 % faster and especially error-free, and the word-finding difficulties have also noticeably improved.
super general performance capacity ….
the previously necessary midday nap ….. no chance,
since spring we have been working through every day until 10 p.m. with just a 15 min lunch break
We drive to Vienna on Wednesday nights, arrive there at 2 a.m. and work until Saturday night in our Vienna Brain Center, arrive back home on Sunday at 2 a.m. in order to then write lectures, articles or instructions on Sunday – or do the bookkeeping.
Only break for the whole year was last week 3 days in the mountains.
Yet this year I planned to retire – Lenna will officially go into full retirement in 2 months, currently she is in “early retirement”
instead we “started new” our beautiful Vienna clinic.
Only possible using Senolysis, TPS, tDCS, Peptides – I feel younger than with age 40 – about 25 years ago !!! Actually, I also look younger than back then!
in this article here I have worked out the background of the peptides
I am especially interested in SEMAX because of its neuroregenerative activity
Therefore the following research on stability.
they told us that peptides do not stay stable even in lyophilized state
“… because if you open the fridge door and they warm up even slightly, they fall apart even in the lyophilized state …. was explained to us during the peptide training“.
Now this contradicts everything we used to do in the research lab, where we used diluted and sterile-filtered albumin solutions for months in the fridge, or lactoferrin or other proteins that we prepared ourselves.
This is how the Russians do it with SEMAX
The Russian pharmaceutical form is remarkably simple. The currently registered SEMAX 0.1 % nasal product from Peptogen contains per liter:
- SEMAX 1 g
- Methylparahydroxybenzoate, Nipagin, 1 g
- purified water ad 1 liter
So converted:
- SEMAX 1 mg/ml
- Methylparaben 1 mg/ml = 0.1 %
- purified water
There is no classical phosphate, citrate or acetate buffer indicated, also no benzyl alcohol and no NaCl. (Vidal Russia)
The decisive point
The Russians therefore apparently do not stabilize SEMAX by means of a complicated buffer, but essentially by:
SEMAX + 0.1 % methylparaben + water + refrigerated storage.
The official Russian medicinal product information states storage of the preparation at 2-8 degrees C. (Vidal Russia)
This also matches the manufacturer information: SEMAX contains a preservative and can therefore remain at room temperature for a few days; for longer storage, however, the manufacturer recommends the refrigerator. (Semax)
This practically refutes the claim …..
“SEMAX falls apart immediately as soon as it is dissolved”
practically refuted.
The Russian finished medicinal product is already an aqueous SEMAX solution, is sold as a multi-dose bottle and possesses pharmaceutical shelf life. It can therefore obviously be formulated sufficiently stably in the aqueous phase. (Vidal Russia)
Interestingly, a slightly acidic pH range of around pH 4.0-5.5 is described for aqueous SEMAX. This could be an essential part of the chemical stability, even if the current specialist information does not list a separate buffer. (medbiol.ru)
If one wanted to replicate the Russian formulation
For an intranasal pharmaceutical formulation the Russian principle would therefore be:
SEMAX 1 mg/ml + methylparaben 1 mg/ml + purified water, refrigerated at 2-8 degrees C.
This is considerably closer to the original than bacteriostatic water with 0.9 % benzyl alcohol. Benzyl alcohol is precisely not contained in the Russian SEMAX recipe. (Vidal Russia)
An important caveat: I would not automatically derive a recipe for sterile injection from this. The Russian formulation is explicitly intranasal, and the requirements for sterility, endotoxins, osmolality and excipients are different for parenteral applications.
Even more interesting would be the original development
I can go even deeper into the Russian SEMAX patents and pharmaceutical development work. There one might find why exactly 0.1 % Nipagin, which pH range and which measured residual SEMAX concentration after months were chosen. That would be the actual answer to the question of how SEMAX is chemically maximally stabilized.
Methylparaben in Semax – any effect other than bactericidal action?
Primarily methylparaben is a preservative. It is intended to prevent the growth of bacteria and especially yeasts/fungi in the aqueous multi-dose solution. A relevant direct chemical stabilization of the SEMAX peptide by methylparaben I would not assume.
What it achieves indirectly
Methylparaben can improve shelf life indirectly:
- prevents microbial degradation of the peptide
- prevents pH changes caused by microorganisms
- enables the repeated use of an aqueous multi-dose bottle
But it does not specifically prevent the typical chemical degradation pathways of a peptide such as oxidation, hydrolysis or deamidation.
The interesting thing about the Russian recipe
This leaves the actually exciting question:
Why is SEMAX nevertheless sufficiently stable in the Russian aqueous solution?
The more probable explanation is that SEMAX itself in clean water at a suitable temperature is considerably more stable than the claim “immediately unstable after reconstitution” would suggest.
The Russian finished formulation is a fairly strong practical counter-argument for this: It consists essentially of SEMAX + water + antimicrobial preservative, without an obvious classical stabilization system.
I would therefore functionally classify methylparaben as follows:
Methylparaben = microbial preservation, not actual peptide stabilization.
For the chemical stability of SEMAX, temperature, pH, oxygen/light and possibly the oxidation of the N-terminal methionine are presumably considerably more interesting.
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